Resources
3D printer files for embryo/tadpole stamps
Tadpole stamp (X. tropicalis, stages 44-47)
Embryos stamp (X. tropicalis, stages 1-20)
Tadpole stamp (X. laevis, stages 44-47)
3D printer files for ISH/IF basket racks
Small rack (fits 24 baskets), for glass dish use #900170 from Wheaton Inc.
Medium rack (fits 30 baskets), for glass dish use #900203 from Wheaton Inc.
Large rack (fits 60 baskets), for glass dish use #49WF37 from Grainger
Making Baskets Protocol, we use 200um mesh for IF (Spectra Mesh #146487) and 100um mesh for ISH (Spectra Mesh #146488)
RNA-Seq from dissected X. tropicalis brains over development
Clonotator
Clonotator is a web-based platform made by the Jeremy Willsey lab that integrates several bioinformatics tools to facilitate efficient screening and annotation of cDNA construct sequences for use in molecular biological experiments. Clonotator automatically translates the nucleotide sequence of the construct into an amino acid sequence, aligns the predicted sequence to a reference database of protein sequences and identifies the best protein and isoform match, annotates any variants present in the construct, and incorporates disease-associated mutations and transcriptomic data to help the user assess suitability for a particular biological context (e.g., isoform affected by disease-associated variant, isoform expressed in tissue of interest).