Clonotator

Clonotator is a web-based platform made by the Jeremy Willsey lab that integrates several bioinformatics tools to facilitate efficient screening and annotation of cDNA construct sequences for use in molecular biological experiments. Clonotator automatically translates the nucleotide sequence of the construct into an amino acid sequence, aligns the predicted sequence to a reference database of protein sequences and identifies the best protein and isoform match, annotates any variants present in the construct, and incorporates disease-associated mutations and transcriptomic data to help the user assess suitability for a particular biological context (e.g., isoform affected by disease-associated variant, isoform expressed in tissue of interest).